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Immunohistochemical staining of human heart muscle shows strong cytoplasmic positivity in myocytes.
Immunohistochemical staining of human heart muscle shows strong cytoplasmic positivity in myocytes.
Immunohistochemical staining of human heart muscle shows strong cytoplasmic positivity in myocytes.

Anti-ICA1 Antibody

HPA017646
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetICA1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-ICA1
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID3382
  • Target name
    ICA1
  • Target description
    islet cell autoantigen 1
  • Target synonyms
    ICA69, ICAp69, islet cell autoantigen 1, 69 kDa islet cell autoantigen, diabetes mellitus type I autoantigen, islet cell autoantigen 1, 69kDa, islet cell autoantigen p69, p69, testicular tissue protein Li 162
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ05084
  • Protein Name
    Islet cell autoantigen 1
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of ICA1 using anti-ICA1 antibody (PB9495). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SH-SY5Y whole cell lysates, Lane 2: human RT4 whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human K562 whole cell lysates, Lane 5: rat pancreas tissue lysates, Lane 6: rat brain tissue lysates, Lane 7: mouse pancreas tissue lysates, Lane 8: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ICA1 antigen affinity purified polyclonal antibody (Catalog # PB9495) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ICA1 at approximately 60-65 kDa. The expected band size for ICA1 is at 55 kDa.
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