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Western blot All lanes: ICA1 antibody at 2microg/ml + A549 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 55, 58 kDa Observed band size: 55 kDa
Western blot All lanes: ICA1 antibody at 2microg/ml + A549 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 55, 58 kDa Observed band size: 55 kDa
Western blot All lanes: ICA1 antibody at 2microg/ml + A549 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 55, 58 kDa Observed band size: 55 kDa

ICA1 Antibody

CSB-PA12109A0RB
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetICA1
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Overview

  • Supplier
    Cusabio
  • Product Name
    ICA1 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID3382
  • Target name
    ICA1
  • Target description
    islet cell autoantigen 1
  • Target synonyms
    69 kDa islet cell autoantigen; diabetes mellitus type I autoantigen; ICA69; ICAp69; islet cell autoantigen 1; islet cell autoantigen 1, 69kDa; islet cell autoantigen p69; p69; testicular tissue protein Li 162
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ05084
  • Protein Name
    Islet cell autoantigen 1
  • Scientific Description
    May play a role in neurotransmitter secretion
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of ICA1 using anti-ICA1 antibody (PB9495). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SH-SY5Y whole cell lysates, Lane 2: human RT4 whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human K562 whole cell lysates, Lane 5: rat pancreas tissue lysates, Lane 6: rat brain tissue lysates, Lane 7: mouse pancreas tissue lysates, Lane 8: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ICA1 antigen affinity purified polyclonal antibody (Catalog # PB9495) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ICA1 at approximately 60-65 kDa. The expected band size for ICA1 is at 55 kDa.
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