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Figure 1. IHC analysis of ICAM1 using anti-ICAM1 antibody (PB9017). ICAM1 was detected in paraffin-embedded section of human intestinal cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1microg/ml rabbit anti-ICAM1 Antibody (PB9017) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Figure 1. IHC analysis of ICAM1 using anti-ICAM1 antibody (PB9017). ICAM1 was detected in paraffin-embedded section of human intestinal cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1microg/ml rabbit anti-ICAM1 Antibody (PB9017) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Figure 1. IHC analysis of ICAM1 using anti-ICAM1 antibody (PB9017). ICAM1 was detected in paraffin-embedded section of human intestinal cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1microg/ml rabbit anti-ICAM1 Antibody (PB9017) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Anti-ICAM-1 Antibody

PB9017
Boster Bio
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen
Product group Antibodies
ReactivityHuman
TargetICAM1
100 ug
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Overview

  • Supplier
    Boster Bio
  • Product Name
    Anti-ICAM-1 Antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen
  • Applications Supplier
    IHP, WB, IHC
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    500 ug/ml
  • Gene ID3383
  • Target name
    ICAM1
  • Target description
    intercellular adhesion molecule 1
  • Target synonyms
    BB2, CD54, P3.58, intercellular adhesion molecule 1, ICAM-1, cell surface glycoprotein P3.58, epididymis secretory sperm binding protein, intercellular adhesion molecule 1 (CD54), human rhinovirus receptor, major group rhinovirus receptor
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP05362
  • Protein Name
    Intercellular adhesion molecule 1
  • Scientific Description
    Boster Bio Anti-ICAM1 Antibody Picoband® catalog # PB9017. Tested in Flow Cytometry, IF, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
  • Reactivity
    Human
  • Reactivity Supplier
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Wang Y, Travers RJ, Farrell A, et al. Differential vascular endothelial cell toxicity of established and novel BCR-ABL tyrosine kinase inhibitors. PLoS One. 2023,18(11):e0294438. doi: 10.1371/journal.pone.0294438
    Read this paper
  • Ma G, Pan B, Ren S, et al. 15-oxoeicosatetraenoic acid mediates monocyte adhesion to endothelial cell. Lipids Health Dis. 2017,16(1):137. doi: 10.1186/s12944-017-0518-2
    Read this paper
  • Jiang Q, Song X, Chen Z, et al. Effects of remifentanil on hemodynamics, liver function and ICAM-1 expression in liver cancer patients undergoing surgery. Oncol Lett. 2017,14(1):872-876. doi: 10.3892/ol.2017.6247
    Read this paper
  • Huang WY, Fu L, Li CY, et al. Quercetin, Hyperin, and Chlorogenic Acid Improve Endothelial Function by Antioxidant, Antiinflammatory, and ACE Inhibitory Effects. J Food Sci. 2017,82(5):1239-1246. doi: 10.1111/1750-3841.13706
    Read this paper
  • Ma G, Pan B, Chen Y, et al. Trimethylamine N-oxide in atherogenesis: impairing endothelial self-repair capacity and enhancing monocyte adhesion. Biosci Rep. 2017,37(2):pii: BSR20160244. doi: 10.1042/BSR20160244.
    Read this paper
  • Huang KF, Huang XP, Xiao GQ, et al. Kallistatin, a novel anti-angiogenesis agent, inhibits angiogenesis via inhibition of the NF-κB signaling pathway. Biomed Pharmacother. 2014,68(4):455-61. doi: 10.1016/j.biopha.2014.03.005
    Read this paper
  • Feng L, Zhu M, Zhang M, et al. Amelioration of compound 4,4'-diphenylmethane-bis(methyl)carbamate on high mobility group box1-mediated inflammation and oxidant stress responses in human umbilical vein endothelial cells via RAGE/ERK1/2/NF-κB pathway. Int Immunopharmacol. 2013,15(2):206-16. doi: 10.1016/j.intimp.2012.11.015
    Read this paper
  • Feng L, Xu YH, Wang SS, et al. Preventative effects of 4,4'-diphenylmethane-bis(methyl) carbamate isolated from cortex mori on human umbilical vein endothelial cell dysfunction induced by advanced glycation end products. Phytother Res. 2012,26(3):412-9. doi: 10.1002/ptr.3569
    Read this paper
  • Xu Y, Feng L, Wang S, et al. Phytoestrogen calycosin-7-O-β-D-glucopyranoside ameliorates advanced glycation end products-induced HUVEC damage. J Cell Biochem. 2011,112(10):2953-65. doi: 10.1002/jcb.23212
    Read this paper
  • Huang YL, Kou JP, Ma L, et al. Possible mechanism of the anti-inflammatory activity of ruscogenin: role of intercellular adhesion molecule-1 and nuclear factor-kappaB. J Pharmacol Sci. 2008,108(2):198-205.
    Read this paper

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Figure 1. Western blot analysis of ICAM1 using anti-ICAM1 antibody (A00171-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Ramos whole cell lysates, Lane 2: human Raji whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ICAM1 antigen affinity purified polyclonal antibody (Catalog # A00171-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ICAM1 at approximately 95 kDa. The expected band size for ICAM1 is at 95 kDa.
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