![Untreated (–) and treated (+) A549 whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ICAM1 / CD54 antibody [N1C2] (GTX100450) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. Untreated (–) and treated (+) A549 whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ICAM1 / CD54 antibody [N1C2] (GTX100450) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](https://www.genetex.com/upload/website/prouct_img/normal/GTX100450/GTX100450_40086_20220701_WB_treatment_hIFN-alpha_22070501_422.webp)
Untreated (–) and treated (+) A549 whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ICAM1 / CD54 antibody [N1C2] (GTX100450) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
ICAM1 / CD54 antibody [N1C2]
GTX100450
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Rat
TargetICAM1
Overview
- SupplierGeneTex
- Product NameICAM1 / CD54 antibody [N1C2]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID3383
- Target nameICAM1
- Target descriptionintercellular adhesion molecule 1
- Target synonymsBB2, CD54, P3.58, intercellular adhesion molecule 1, ICAM-1, cell surface glycoprotein P3.58, epididymis secretory sperm binding protein, intercellular adhesion molecule 1 (CD54), human rhinovirus receptor, major group rhinovirus receptor
- HostRabbit
- IsotypeIgG
- Protein IDP05362
- Protein NameIntercellular adhesion molecule 1
- Scientific DescriptionThis gene encodes a cell surface glycoprotein which is typically expressed on endothelial cells and cells of the immune system. It binds to integrins of type CD11a / CD18, or CD11b / CD18 and is also exploited by Rhinovirus as a receptor. [provided by RefSeq]
- ReactivityHuman, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC41116161

![ICAM1 / CD54 antibody [N1C2] detects ICAM1 / CD54 protein at cell membrane by immunohistochemical analysis. Sample: Paraffin-embedded human lung cancer. Green: ICAM1 / CD54 stained by ICAM1 / CD54 antibody [N1C2] (GTX100450) diluted at 1:250. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000. Blue: Fluoroshield with DAPI (GTX30920). Antigen Retrieval: Citrate buffer, pH 6.0, 15 min ICAM1 / CD54 antibody [N1C2] detects ICAM1 / CD54 protein at cell membrane by immunohistochemical analysis. Sample: Paraffin-embedded human lung cancer. Green: ICAM1 / CD54 stained by ICAM1 / CD54 antibody [N1C2] (GTX100450) diluted at 1:250. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000. Blue: Fluoroshield with DAPI (GTX30920). Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX100450/GTX100450_40086_20191108_IHC-P-FL_w_23060100_975.webp)








