Untreated (–) and treated (+) A549 whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ICAM1 / CD54 antibody [N1C2] (GTX100450) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
![ICAM1 / CD54 antibody [N1C2] detects ICAM1 / CD54 protein at cell membrane by immunohistochemical analysis. Sample: Paraffin-embedded human lung cancer. Green: ICAM1 / CD54 stained by ICAM1 / CD54 antibody [N1C2] (GTX100450) diluted at 1:250. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000. Blue: Fluoroshield with DAPI (GTX30920). Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX100450/GTX100450_40086_20191108_IHC-P-FL_w_23060100_975.webp "ICAM1 / CD54 antibody [N1C2] detects ICAM1 / CD54 protein at cell membrane by immunohistochemical analysis. Sample: Paraffin-embedded human lung cancer. Green: ICAM1 / CD54 stained by ICAM1 / CD54 antibody [N1C2] (GTX100450) diluted at 1:250. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000. Blue: Fluoroshield with DAPI (GTX30920). Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
Untreated (–) and treated (+) A549 whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ICAM1 / CD54 antibody [N1C2] (GTX100450) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
ICAM1 / CD54 antibody [N1C2]
GTX100450
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Rat
TargetICAM1
Overview
- SupplierGeneTex
- Product NameICAM1 / CD54 antibody [N1C2]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID3383
- Target nameICAM1
- Target descriptionintercellular adhesion molecule 1
- Target synonymsBB2, CD54, P3.58, intercellular adhesion molecule 1, ICAM-1, cell surface glycoprotein P3.58, epididymis secretory sperm binding protein, intercellular adhesion molecule 1 (CD54), human rhinovirus receptor, major group rhinovirus receptor
- HostRabbit
- IsotypeIgG
- Protein IDP05362
- Protein NameIntercellular adhesion molecule 1
- Scientific DescriptionThis gene encodes a cell surface glycoprotein which is typically expressed on endothelial cells and cells of the immune system. It binds to integrins of type CD11a / CD18, or CD11b / CD18 and is also exploited by Rhinovirus as a receptor. [provided by RefSeq]
- ReactivityHuman, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- Shi YJ, Yang CG, Qiao WB, et al. Sacubitril/valsartan attenuates myocardial inflammation, hypertrophy, and fibrosis in rats with heart failure with preserved ejection fraction. Eur J Pharmacol. 2023,961:176170. doi: 10.1016/j.ejphar.2023.176170Read this paper
- Vos S, Aaron R, Weng M, et al. CD40 Upregulation in the Retina of Patients With Diabetic Retinopathy: Association With TRAF2/TRAF6 Upregulation and Inflammatory Molecule Expression. Invest Ophthalmol Vis Sci. 2023,64(7):17. doi: 10.1167/iovs.64.7.17Read this paper
- Wu CY, Peng PW, Renn TY, et al. CX3CL1 induces cell migration and invasion through ICAM-1 expression in oral squamous cell carcinoma cells. J Cell Mol Med. 2023,27(11):1509-1522. doi: 10.1111/jcmm.17750Read this paper
- Chang TM, Chiang YC, Lee CW, et al. CXCL14 promotes metastasis of non-small cell lung cancer through ACKR2-depended signaling pathway. Int J Biol Sci. 2023,19(5):1455-1470. doi: 10.7150/ijbs.79438Read this paper
- Chang MC, Wang TM, Chien HH, et al. Effect of butyrate, a bacterial by-product, on the viability and ICAM-1 expression/production of human vascular endothelial cells: Role in infectious pulpal/periapical diseases. Int Endod J. 2022,55(1):38-53. doi: 10.1111/iej.13614Read this paper
- Chang WC, Wu JS, Shen SC. Vescalagin from Pink Wax Apple (Syzygium samarangense (Blume) Merrill and Perry) Protects Pancreatic β-Cells against Methylglyoxal-Induced Inflammation in Rats. Plants (Basel). 2021,10(7). doi: 10.3390/plants10071448Read this paper
- Corgnac S, Malenica I, Mezquita L, et al. CD103(+)CD8(+) T(RM) Cells Accumulate in Tumors of Anti-PD-1-Responder Lung Cancer Patients and Are Tumor-Reactive Lymphocytes Enriched with Tc17. Cell Rep Med. 2020,1(7):100127. doi: 10.1016/j.xcrm.2020.100127Read this paper
- Solé M, Esteban-Lopez M, Taltavull B, et al. Blood-brain barrier dysfunction underlying Alzheimer's disease is induced by an SSAO/VAP-1-dependent cerebrovascular activation with enhanced Aβ deposition. Biochim Biophys Acta Mol Basis Dis. 2019,1865(9):2189-2202. doi: 10.1016/j.bbadis.2019.04.016Read this paper
- Huang FI, Wu YW, Sung TY, et al. MPT0G413, A Novel HDAC6-Selective Inhibitor, and Bortezomib Synergistically Exert Anti-tumor Activity in Multiple Myeloma Cells. Front Oncol. 2019,9:249. doi: 10.3389/fonc.2019.00249Read this paper
- Chang MC, Lin SI, Pan YH, et al. IL-1β-induced ICAM-1 and IL-8 expression/secretion of dental pulp cells is differentially regulated by IRAK and p38. J Formos Med Assoc. 2019,118(8):1247-1254. doi: 10.1016/j.jfma.2018.11.015Read this paper
Datasheet
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