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ProductsBack/ICAM1 / CD54 antibody [N1C2]

ICAM1 / CD54 antibody [N1C2]

GTX100450
GeneTex
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetICAM1
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Overview

  • Supplier
    GeneTex
  • Product Name
    ICAM1 / CD54 antibody [N1C2]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID3383
  • Target name
    ICAM1
  • Target description
    intercellular adhesion molecule 1
  • Target synonyms
    BB2, CD54, P3.58, intercellular adhesion molecule 1, ICAM-1, cell surface glycoprotein P3.58, epididymis secretory sperm binding protein, intercellular adhesion molecule 1 (CD54), human rhinovirus receptor, major group rhinovirus receptor
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP05362
  • Protein Name
    Intercellular adhesion molecule 1
  • Scientific Description
    This gene encodes a cell surface glycoprotein which is typically expressed on endothelial cells and cells of the immune system. It binds to integrins of type CD11a / CD18, or CD11b / CD18 and is also exploited by Rhinovirus as a receptor. [provided by RefSeq]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Shi YJ, Yang CG, Qiao WB, et al. Sacubitril/valsartan attenuates myocardial inflammation, hypertrophy, and fibrosis in rats with heart failure with preserved ejection fraction. Eur J Pharmacol. 2023,961:176170. doi: 10.1016/j.ejphar.2023.176170
    Read this paper
  • Vos S, Aaron R, Weng M, et al. CD40 Upregulation in the Retina of Patients With Diabetic Retinopathy: Association With TRAF2/TRAF6 Upregulation and Inflammatory Molecule Expression. Invest Ophthalmol Vis Sci. 2023,64(7):17. doi: 10.1167/iovs.64.7.17
    Read this paper
  • Wu CY, Peng PW, Renn TY, et al. CX3CL1 induces cell migration and invasion through ICAM-1 expression in oral squamous cell carcinoma cells. J Cell Mol Med. 2023,27(11):1509-1522. doi: 10.1111/jcmm.17750
    Read this paper
  • Chang TM, Chiang YC, Lee CW, et al. CXCL14 promotes metastasis of non-small cell lung cancer through ACKR2-depended signaling pathway. Int J Biol Sci. 2023,19(5):1455-1470. doi: 10.7150/ijbs.79438
    Read this paper
  • Chang MC, Wang TM, Chien HH, et al. Effect of butyrate, a bacterial by-product, on the viability and ICAM-1 expression/production of human vascular endothelial cells: Role in infectious pulpal/periapical diseases. Int Endod J. 2022,55(1):38-53. doi: 10.1111/iej.13614
    Read this paper
  • Chang WC, Wu JS, Shen SC. Vescalagin from Pink Wax Apple (Syzygium samarangense (Blume) Merrill and Perry) Protects Pancreatic β-Cells against Methylglyoxal-Induced Inflammation in Rats. Plants (Basel). 2021,10(7). doi: 10.3390/plants10071448
    Read this paper
  • Corgnac S, Malenica I, Mezquita L, et al. CD103(+)CD8(+) T(RM) Cells Accumulate in Tumors of Anti-PD-1-Responder Lung Cancer Patients and Are Tumor-Reactive Lymphocytes Enriched with Tc17. Cell Rep Med. 2020,1(7):100127. doi: 10.1016/j.xcrm.2020.100127
    Read this paper
  • Solé M, Esteban-Lopez M, Taltavull B, et al. Blood-brain barrier dysfunction underlying Alzheimer's disease is induced by an SSAO/VAP-1-dependent cerebrovascular activation with enhanced Aβ deposition. Biochim Biophys Acta Mol Basis Dis. 2019,1865(9):2189-2202. doi: 10.1016/j.bbadis.2019.04.016
    Read this paper
  • Huang FI, Wu YW, Sung TY, et al. MPT0G413, A Novel HDAC6-Selective Inhibitor, and Bortezomib Synergistically Exert Anti-tumor Activity in Multiple Myeloma Cells. Front Oncol. 2019,9:249. doi: 10.3389/fonc.2019.00249
    Read this paper
  • Chang MC, Lin SI, Pan YH, et al. IL-1β-induced ICAM-1 and IL-8 expression/secretion of dental pulp cells is differentially regulated by IRAK and p38. J Formos Med Assoc. 2019,118(8):1247-1254. doi: 10.1016/j.jfma.2018.11.015
    Read this paper

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Figure 1. Western blot analysis of ICAM1 using anti-ICAM1 antibody (A00171-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Ramos whole cell lysates, Lane 2: human Raji whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ICAM1 antigen affinity purified polyclonal antibody (Catalog # A00171-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ICAM1 at approximately 95 kDa. The expected band size for ICAM1 is at 95 kDa.
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