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Immunohistochemical staining of human cerebral cortex shows distinct positivity in neuropil.
Immunohistochemical staining of human cerebral cortex shows distinct positivity in neuropil.
Immunohistochemical staining of human cerebral cortex shows distinct positivity in neuropil.

Anti-ICAM5 Antibody

HPA009083
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetICAM5
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-ICAM5
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID7087
  • Target name
    ICAM5
  • Target description
    intercellular adhesion molecule 5
  • Target synonyms
    TLCN, TLN, intercellular adhesion molecule 5, ICAM-5, intercellular adhesion molecule 5, telencephalin, telencephalin
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9UMF0
  • Protein Name
    Intercellular adhesion molecule 5
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of ICAM5 using anti-ICAM5 antibody (A07187-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ICAM5 antigen affinity purified polyclonal antibody (Catalog # A07187-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ICAM5 at approximately 140 kDa. The expected band size for ICAM5 is at 97 kDa.
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