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Immunohistochemical staining of human cerebellum shows moderate cytoplasm granular positivity in Purkinje cells.
Immunohistochemical staining of human cerebellum shows moderate cytoplasm granular positivity in Purkinje cells.
Immunohistochemical staining of human cerebellum shows moderate cytoplasm granular positivity in Purkinje cells.

Anti-ICMT Antibody

HPA032025
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
TargetICMT
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-ICMT
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID23463
  • Target name
    ICMT
  • Target description
    isoprenylcysteine carboxyl methyltransferase
  • Target synonyms
    HSTE14; MST098; MSTP098; PCCMT; PCMT; PPMT; prenylated protein carboxyl methyltransferase; prenylcysteine carboxyl methyltransferase; protein-S-isoprenylcysteine O-methyltransferase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO60725
  • Protein Name
    Protein-S-isoprenylcysteine O-methyltransferase
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of ICMT using anti-ICMT antibody (A06174-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: rat C6 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ICMT antigen affinity purified polyclonal antibody (Catalog # A06174-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ICMT at approximately 32 kDa. The expected band size for ICMT is at 32 kDa.
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Paraformaldehyde-fixed, paraffin-embedded Mouse Brain tissue; Antigen retrieval by boiling in sodium citrate buffer (0.01M, pH6) for 15 minutes; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20 minutes; Antibody incubation with ICMT Polyclonal Antibody, Unconjugated (bs-8019R) at 1:400 overnight at 4°C, followed by incubation with a conjugated secondary antibody, DAB and counterstaining.
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Immunohistochemical staining of human cerebellum shows moderate cytoplasmic positivity in Purkinje cells.
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