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ICMT antibody

GTX129471
GeneTex
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetICMT
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Overview

  • Supplier
    GeneTex
  • Product Name
    ICMT antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID23463
  • Target name
    ICMT
  • Target description
    isoprenylcysteine carboxyl methyltransferase
  • Target synonyms
    HSTE14; MST098; MSTP098; PCCMT; PCMT; PPMT; prenylated protein carboxyl methyltransferase; prenylcysteine carboxyl methyltransferase; protein-S-isoprenylcysteine O-methyltransferase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO60725
  • Protein Name
    Protein-S-isoprenylcysteine O-methyltransferase
  • Scientific Description
    This gene encodes the third of three enzymes that posttranslationally modify isoprenylated C-terminal cysteine residues in certain proteins and target those proteins to the cell membrane. This enzyme localizes to the endoplasmic reticulum. Alternative splicing may result in other transcript variants, but the biological validity of those transcripts has not been determined. [provided by RefSeq]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Licoricidin combats gastric cancer by targeting the ICMT/Ras pathway in vitro and in vivo.
    Read more

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Figure 1. Western blot analysis of ICMT using anti-ICMT antibody (A06174-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: rat C6 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ICMT antigen affinity purified polyclonal antibody (Catalog # A06174-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ICMT at approximately 32 kDa. The expected band size for ICMT is at 32 kDa.
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