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Immunohistochemical staining of human parathyroid gland shows strong granular cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human parathyroid gland shows strong granular cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human parathyroid gland shows strong granular cytoplasmic positivity in glandular cells.

Anti-IDH3B Antibody

HPA049387
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
ReactivityHuman
TargetIDH3B
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-IDH3B
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID3420
  • Target name
    IDH3B
  • Target description
    isocitrate dehydrogenase (NAD(+)) 3 non-catalytic subunit beta
  • Target synonyms
    isocitrate dehydrogenase (NAD(+)) 3 beta; isocitrate dehydrogenase [NAD] subunit beta, mitochondrial; isocitrate dehydrogenase 3 (NAD(+)) beta; isocitrate dehydrogenase 3 (NAD+) beta; isocitric dehydrogenase subunit beta; NAD(+)-specific ICDH subunit beta; RP46
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO43837
  • Protein Name
    Isocitrate dehydrogenase [NAD] subunit beta, mitochondrial
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Western blot All lanes: IDH3B antibody at 2microg/ml Lane 1: Hela whole cell lysate Lane 2: Mouse kidney tissue Lane 3: Mouse gonadal tissue Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 43, 42, 26 kDa Observed band size: 43 kDa
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Figure 1. Western blot analysis of IDH3B using anti-IDH3B antibody (A09433-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human LANAP whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human RT4 whole cell lysates, Lane 4: human U251 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat heart tissue lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IDH3B antigen affinity purified polyclonal antibody (Catalog # A09433-3) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IDH3B at approximately 42 kDa. The expected band size for IDH3B is at 42 kDa.
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WB analysis of HeLa (A), Jurkat (B), PC12 (C) whole cell lysates using GTX56056 IDH3B antibody.
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