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IDH3B Antibody

LS-C748773
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
TargetIDH3B
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    IDH3B Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 25kDa/41kDa/42kDa, while the observed MW by Western blot was 42kDa.
  • Applications
    Western Blot
  • Applications Supplier
    WB (1:500 - 1:2000) The predicted MW is 25kDa/41kDa/42kDa, while the observed MW by Western blot was 42kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID3420
  • Target name
    IDH3B
  • Target description
    isocitrate dehydrogenase (NAD(+)) 3 non-catalytic subunit beta
  • Target synonyms
    RP46, isocitrate dehydrogenase [NAD] subunit beta, mitochondrial, NAD(+)-specific ICDH subunit beta, isocitrate dehydrogenase (NAD(+)) 3 beta, isocitrate dehydrogenase 3 (NAD(+)) beta, isocitrate dehydrogenase 3 (NAD+) beta, isocitric dehydrogenase subunit beta
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of IDH3B using anti-IDH3B antibody (A09433-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human LANAP whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human RT4 whole cell lysates, Lane 4: human U251 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat heart tissue lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IDH3B antigen affinity purified polyclonal antibody (Catalog # A09433-3) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IDH3B at approximately 42 kDa. The expected band size for IDH3B is at 42 kDa.
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