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Anti-IDO2 Antibody

HY-P990437
MedChem Express
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Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    Anti-IDO2 Antibody
  • Delivery Days Customer
    5
  • Certification
    Research Use Only
  • Scientific Description
    Anti-IDO2 Antibody is a CHO-expressed human antibody that targets IDO2. Anti-IDO2 Antibody has a huIgG4SP heavy chain and a hukappa light chain, with a predicted molecular weight (MW) of 151.06 kDa. The isotype control for Anti-IDO2 Antibody can refer to Human IgG1 kappa, Isotype Control (HY-P99001).
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of IDO2 using anti-IDO2 antibody (PA1612). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates (positive control), Lane 2: human placenta tissue lysates (positive control), Lane 3: human Caco-2 whole cell lysates (positive control), Lane 4: human PC-3 whole cell lysates (negative control). After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IDO2 antigen affinity purified polyclonal antibody (Catalog # PA1612) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IDO2 at approximately 47KD. The expected band size for IDO2 is at 47KD.
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