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Western Blot analysis of HeLa HepG2 cells using INDOL1 Polyclonal Antibody
Western Blot analysis of HeLa HepG2 cells using INDOL1 Polyclonal Antibody
Western Blot analysis of HeLa HepG2 cells using INDOL1 Polyclonal Antibody

IDO2 Antibody

CSB-PA006016
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetIDO2
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Overview

  • Supplier
    Cusabio
  • Product Name
    IDO2 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID169355
  • Target name
    IDO2
  • Target description
    indoleamine 2,3-dioxygenase 2
  • Target synonyms
    INDOL1; indoleamine 2,3-dioxygenase 2; indoleamine 2,3-dioxygenase-like 1 protein; indoleamine 2,3-dioxygenase-like protein 1; indoleamine-pyrrole 2,3 dioxygenase-like 1; indoleamine-pyrrole 2,3-dioxygenase-like protein 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ6ZQW0
  • Protein Name
    Indoleamine 2,3-dioxygenase 2
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of IDO2 using anti-IDO2 antibody (PA1612). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates (positive control), Lane 2: human placenta tissue lysates (positive control), Lane 3: human Caco-2 whole cell lysates (positive control), Lane 4: human PC-3 whole cell lysates (negative control). After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IDO2 antigen affinity purified polyclonal antibody (Catalog # PA1612) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IDO2 at approximately 47KD. The expected band size for IDO2 is at 47KD.
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