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Immunohistochemical staining of human urinary bladder shows weak cytoplasmic positivity in urothelial cells.
Immunohistochemical staining of human urinary bladder shows weak cytoplasmic positivity in urothelial cells.
Immunohistochemical staining of human urinary bladder shows weak cytoplasmic positivity in urothelial cells.

Anti-IFI44L Antibody

HPA060372
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
ReactivityHuman
TargetIFI44L
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-IFI44L
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID10964
  • Target name
    IFI44L
  • Target description
    interferon induced protein 44 like
  • Target synonyms
    C1orf29; GS3686; interferon-induced protein 44-like; TLDC5B
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ53G44
  • Protein Name
    Interferon-induced protein 44-like
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Western Blot Positive WB detected in: HepG2 whole cell lysate, A549 whole cell lysate, NIH/3T3 whole cell lysate All lanes: IFI44L antibody at 2.5microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 52, 19 kDa Observed band size: 52 kDa
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Figure 1. Western blot analysis of IFI44L using anti-IFI44L antibody (A10975-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SiHa whole cell lysates, Lane 2: human PC-3 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: monkey COS-7 whole cell lysates, Lane 5: mouse NRK whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IFI44L antigen affinity purified polyclonal antibody (A10975-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IFI44L at approximately 51 kDa. The expected band size for IFI44L is at 51 kDa.
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Anti-IFI44L Antibody Picoband(r)A10975-1-CARRIER-FREE
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Immunofluorescent staining of human cell line PC-3 shows localization to nucleoplasm.
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Immunofluorescent staining of human cell line PC-3 shows localization to nucleoplasm.
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