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Immunohistochemical staining of human caudate shows strong cytoplasmic positivity in astrocytes.
Immunohistochemical staining of human caudate shows strong cytoplasmic positivity in astrocytes.
Immunohistochemical staining of human caudate shows strong cytoplasmic positivity in astrocytes.

Anti-IFT172 Antibody

HPA044893
Atlas Antibodies
ApplicationsImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
TargetIFT172
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-IFT172
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID26160
  • Target name
    IFT172
  • Target description
    intraflagellar transport 172
  • Target synonyms
    BBS20; intraflagellar transport 172 homolog; intraflagellar transport protein 172 homolog; NPHP17; osm-1; RP71; selective LIM binding factor homolog; SLB; SRTD10; wim; wimple homolog
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9UG01
  • Protein Name
    Intraflagellar transport protein 172 homolog
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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IHC image of CSB-PA887019LA01HU diluted at 1:400 and staining in paraffin-embedded human prostate cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Figure 1. Western blot analysis of IFT172 using anti-IFT172 antibody (A08217-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human HEL whole cell lysates, Lane 3: human U251 whole cell lysates, Lane 4: human 293T whole cell lysates, Lane 5: rat testis tissue lysates, Lane 6: rat brain tissue lysates, Lane 7: mouse testis tissue lysates, Lane 8: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IFT172 antigen affinity purified polyclonal antibody (Catalog # A08217-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IFT172 at approximately 180 kDa. The expected band size for IFT172 is at 198 kDa.
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Anti-IFT172 Antibody Picoband(r)A08217-1-CARRIER-FREE
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