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IFT172 Antibody

PACO59501
Assay Genie
ApplicationsImmunoFluorescence, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetIFT172
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Overview

  • Supplier
    Assay Genie
  • Product Name
    IFT172 Antibody
  • Delivery Days Customer
    9
  • Applications
    ImmunoFluorescence, ELISA, ImmunoHistoChemistry
  • Applications Supplier
    ELISA, IHC, IF
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Gene ID26160
  • Target name
    IFT172
  • Target description
    intraflagellar transport 172
  • Target synonyms
    BBS20, NPHP17, RP71, SLB, SRTD10, osm-1, wim, intraflagellar transport protein 172 homolog, intraflagellar transport 172 homolog, selective LIM binding factor homolog, wimple homolog
  • Host
    Rabbit
  • Protein IDQ9UG01
  • Protein Name
    Intraflagellar transport protein 172 homolog
  • Shelf life instruction
    12 months
  • Source
    Rabbit
  • Reactivity
    Human
  • Reactivity Supplier
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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MSDS

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Figure 1. Western blot analysis of IFT172 using anti-IFT172 antibody (A08217-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human HEL whole cell lysates, Lane 3: human U251 whole cell lysates, Lane 4: human 293T whole cell lysates, Lane 5: rat testis tissue lysates, Lane 6: rat brain tissue lysates, Lane 7: mouse testis tissue lysates, Lane 8: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IFT172 antigen affinity purified polyclonal antibody (Catalog # A08217-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IFT172 at approximately 180 kDa. The expected band size for IFT172 is at 198 kDa.
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IHC image of CSB-PA887019LA01HU diluted at 1:400 and staining in paraffin-embedded human prostate cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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