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Immunohistochemical staining of human testis shows strong cytoplasmic positivity in cells in seminiferous ducts.
Immunohistochemical staining of human testis shows strong cytoplasmic positivity in cells in seminiferous ducts.
Immunohistochemical staining of human testis shows strong cytoplasmic positivity in cells in seminiferous ducts.

Anti-IFT43 Antibody

HPA003438
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetIFT43
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-IFT43 Antibody
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID112752
  • Target name
    IFT43
  • Target description
    intraflagellar transport 43
  • Target synonyms
    C14orf179, CED3, RP81, SRTD18, intraflagellar transport protein 43 homolog, IFT complex A subunit, intraflagellar transport 43 homolog
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ96FT9
  • Protein Name
    Intraflagellar transport protein 43 homolog
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of IFT43 using anti-IFT43 antibody (A10264). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human U-87MG whole cell lysates, Lane 3: human T-47D whole cell lysates, Lane 4: human MCF-7 whole cell lysates, Lane 5: human PC-3 whole cell lysates, Lane 6: human A549 whole cell lysates, Lane 7: human Jurkat whole cell lysates, Lane 8: human A431 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IFT43 antigen affinity purified polyclonal antibody (Catalog # A10264) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IFT43 at approximately 23 kDa. The expected band size for IFT43 is at 23 kDa.
Product group Antibodies
Boster Bio
Anti-IFT43 Antibody Picoband(r)A10264-CARRIER-FREE
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
TargetIFT43
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Atlas Antibodies
PrEST Antigen IFT43APREST70660
Protein IDQ96FT9
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Product group Antibodies
LifeSpan BioSciences
IFT43 / C14orf179 AntibodyLS-C664301
TargetIFT43
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Product group Antibodies
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C14orf179 AntibodyABX032498
ApplicationsFlow Cytometry, Western Blot, ELISA, ImmunoHistoChemistry
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