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IFT43 / C14orf179 Antibody

LS-C664301
LifeSpan BioSciences
ReactivityHuman
Product group Antibodies
TargetIFT43
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    IFT43 / C14orf179 Antibody
  • Delivery Days Customer
    23
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID112752
  • Target name
    IFT43
  • Target description
    intraflagellar transport 43
  • Target synonyms
    C14orf179, CED3, RP81, SRTD18, intraflagellar transport protein 43 homolog, IFT complex A subunit, intraflagellar transport 43 homolog
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of IFT43 using anti-IFT43 antibody (A10264). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human U-87MG whole cell lysates, Lane 3: human T-47D whole cell lysates, Lane 4: human MCF-7 whole cell lysates, Lane 5: human PC-3 whole cell lysates, Lane 6: human A549 whole cell lysates, Lane 7: human Jurkat whole cell lysates, Lane 8: human A431 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IFT43 antigen affinity purified polyclonal antibody (Catalog # A10264) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IFT43 at approximately 23 kDa. The expected band size for IFT43 is at 23 kDa.
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