ProductsBack/Anti-IFT74 Antibody

Anti-IFT74 Antibody

144-12672

ApplicationsWestern Blot

Product group Antibodies

TargetIFT74

Overview

Supplier
RayBiotech
Product Name
Anti-IFT74 Antibody
Delivery Days Customer
16
Applications
Western Blot
Certification
Research Use Only
Clonality
Polyclonal
Conjugate
Unconjugated
Gene ID80173
Target name
IFT74
Target description
intraflagellar transport 74
Target synonyms
BBS22; capillary morphogenesis gene 1 protein; capillary morphogenesis protein 1; CCDC2; CMG1; CMG-1; coiled-coil domain containing 2; coiled-coil domain-containing protein 2; intraflagellar transport 74 homolog; intraflagellar transport protein 74 homolog
Host
Rabbit
Isotype
IgG
Protein IDQ96LB3
Protein Name
Intraflagellar transport protein 74 homolog
Scientific Description
IFT74 Polyclonal Antibody
Storage Instruction
-20°C
UNSPSC
12352203

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Figure 1. Western blot analysis of IFT74 using anti-IFT74 antibody (A08931-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human Raji whole cell lysates, Lane 4: monkey COS-7 whole cell lysates, Lane 5: rat thymus tissue lysates, Lane 6: mouse thymus tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IFT74 antigen affinity purified polyclonal antibody (Catalog # A08931-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IFT74 at approximately 69 kDa. The expected band size for IFT74 is at 69 kDa.

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