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Anti-IFT74 Antibody

144-12672
RayBiotech
ApplicationsWestern Blot
Product group Antibodies
TargetIFT74
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Overview

  • Supplier
    RayBiotech
  • Product Name
    Anti-IFT74 Antibody
  • Delivery Days Customer
    16
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID80173
  • Target name
    IFT74
  • Target description
    intraflagellar transport 74
  • Target synonyms
    BBS22, CCDC2, CMG-1, CMG1, JBTS40, SPGF58, intraflagellar transport protein 74 homolog, capillary morphogenesis gene 1 protein, capillary morphogenesis protein 1, coiled-coil domain containing 2, coiled-coil domain-containing protein 2, intraflagellar transport 74 homolog
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ96LB3
  • Protein Name
    Intraflagellar transport protein 74 homolog
  • Scientific Description
    IFT74 Polyclonal Antibody
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of IFT74 using anti-IFT74 antibody (A08931-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human Raji whole cell lysates, Lane 4: monkey COS-7 whole cell lysates, Lane 5: rat thymus tissue lysates, Lane 6: mouse thymus tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IFT74 antigen affinity purified polyclonal antibody (Catalog # A08931-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IFT74 at approximately 69 kDa. The expected band size for IFT74 is at 69 kDa.
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