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The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using CSB-PA040376(IFT74 Antibody) at dilution 1/60, on the right is treated with synthetic peptide. (Original magnification: x200)
The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using CSB-PA040376(IFT74 Antibody) at dilution 1/60, on the right is treated with synthetic peptide. (Original magnification: x200)
The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using CSB-PA040376(IFT74 Antibody) at dilution 1/60, on the right is treated with synthetic peptide. (Original magnification: x200)

IFT74 Antibody

CSB-PA040376
Cusabio
ApplicationsELISA, ImmunoHistoChemistry
Product group Antibodies
TargetIFT74
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Overview

  • Supplier
    Cusabio
  • Product Name
    IFT74 Antibody
  • Delivery Days Customer
    20
  • Applications
    ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID80173
  • Target name
    IFT74
  • Target description
    intraflagellar transport 74
  • Target synonyms
    BBS22; capillary morphogenesis gene 1 protein; capillary morphogenesis protein 1; CCDC2; CMG1; CMG-1; coiled-coil domain containing 2; coiled-coil domain-containing protein 2; intraflagellar transport 74 homolog; intraflagellar transport protein 74 homolog
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ96LB3
  • Protein Name
    Intraflagellar transport protein 74 homolog
  • Scientific Description
    Capillary morphogenesis protein 1 (CMG1), also known as intraflagellar transport protein 74 (ITP74) or coiled-coil domain-containing protein 2 (CCDC2), is a 600 amino acid human homologue of IFT-71, a complex B protein supporting intraflagellar transport (IFT) in Chlamydomonas. CMG1 localizes to the cytoplasmic vesicle and is highly expressed in adult and fetal kidney and testis, with lower levels of expression in adult heart, placenta, lung, liver and pancreas, and in fetal heart, lung and liver. CMG1 has been suggested to have a role in the primary cilia of HUVEC, and it also functions as a transcriptional regulator of cyclin D2 in spermatocyte-derived cells.
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of IFT74 using anti-IFT74 antibody (A08931-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human Raji whole cell lysates, Lane 4: monkey COS-7 whole cell lysates, Lane 5: rat thymus tissue lysates, Lane 6: mouse thymus tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IFT74 antigen affinity purified polyclonal antibody (Catalog # A08931-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IFT74 at approximately 69 kDa. The expected band size for IFT74 is at 69 kDa.
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