Anti-IGF1R [10H5]

Absolute Antibody

Anti-IGF1R [10H5]

9

The orginal humanized IgG1 version of this antibody binds human IGF-1R with an affinity of 1.1 nM in an ELISA. The binding specificity was also comfirmed using fluorescence activated cell sorting of IGF-IR-expressing MCF7 cells. This antibody was also used for the immunoprecipitaion of MCF7 cells. The original antibody inhibits IGF-IR-mediated signaling by blocking IGF-I and IGF-II binding and by inducing cell surface receptor downregulation via internalization and degradation, with the extracellular and intracellular domains of IGF-IR being differentially affected by the proteasomal and lysosomal inhibitors. In vitro, h10H5 exhibits antiproliferative effects on cancer cell lines. In vivo, h10H5 shows single-agent antitumor efficacy in human SK-N-AS neuroblastoma and SW527 breast cancer xenograft models and even greater efficacy in combination with the chemotherapeutic agent docetaxel or an anti-vascular endothelial growth factor antibody (PMID:18790743).

Flow Cytometry, ImmunoPrecipitation, ELISA

Research Use Only

Monoclonal

10H5

IGF1R

insulin like growth factor 1 receptor

CD221, IGFIR, IGFR, JTK13, insulin-like growth factor 1 receptor, IGF-I receptor

Rabbit

IgG

Insulin-like growth factor 1 receptor

This chimeric rabbit antibody was made using the variable domain sequences of the original Human IgG1 format, for improved compatibility with existing reagents, assays and techniques.

Human, Monkey

-20°C,2°C to 8°C

41116161