Anti-IGF1R [IGFR 17-69]

Absolute Antibody

Anti-IGF1R [IGFR 17-69]

9

This antibody is specific for the human insulin-like growth factor 1 receptor (IGF1R). It recognises the extracellular portion of the receptor, specifically an epitope in the alpha subunit (amino acids 514-586). It does not cross-react with rat or mouse I

The ability of this antibody to bind human IGF1R has been confirmed in IGF1 binding assays using both IG1FR-overexpressing IGF1R/3T3 cells and soluble receptors (Soos et al, 1992). This antibody efficiently precipitates human IGF1R (Soos et al, 1992), and has been used in immunoprecipitation analysis of 293 cells transfected with hIGF1R or chimeric hIGF1R-insulin receptor constructs (Schumacher et al, 1993) and solubilized microsomal membrane samples prepared from a range of human tissues (Bailyes et al, 1997). Given its reactivity with rabbit IGF1R, this antibody has also been used in immunoprecipitation analysis of rabbit tissue samples (Bailyes et al, 1997). When immobilised, this antibody has been shown to remove essentially all soluble, IGF1R/3T3-derived IGF1R (Soos et al, 1992) and human placental membrane-derived IGF1R in immunodepletion experiments (Soos et al, 1993). This antibody weakly inhibits IGF1 binding to its receptor (Soos et al, 1993).

ImmunoPrecipitation, Neutralisation/Blocking

Research Use Only

Monoclonal

IGFR 17-69

IGF1R

insulin like growth factor 1 receptor

CD221; IGF-I receptor; IGFIR; IGFR; insulin-like growth factor 1 receptor; JTK13; soluble IGF1R variant 1; soluble IGF1R variant 2

Human

IgG1

Insulin-like growth factor 1 receptor

This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.

Human, Porcine, Rabbit

-20°C,2°C to 8°C

12352203