Western blot analysis of IGF2 expression in human serum lysate (M00335). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IGF2 monoclonal antibody (Catalog # M00335) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IGF2
Anti-IGF2/IGF II Rabbit Monoclonal Antibody
M00335
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman
TargetIGF2
Overview
- SupplierBoster Bio
- Product NameAnti-IGF2/Igf Ii Rabbit Monoclonal Antibody
- Delivery Days Customer9
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDIGE-9
- FormulationLiquid
- Gene ID3481
- Target nameIGF2
- Target descriptioninsulin like growth factor 2
- Target synonymsC11orf43; GRDF; IGF-II; insulin-like growth factor 2 (somatomedin A); insulin-like growth factor II; insulin-like growth factor type 2; PP9974; preptin; SRS3; T3M-11-derived growth factor
- HostRabbit
- IsotypeIgG
- Protein IDP01344
- Protein NameInsulin-like growth factor II
- Scientific DescriptionBoster Bio Anti-IGF2/IGF II Rabbit Monoclonal Antibody catalog # M00335. Tested in WB, ICC/IF applications. This antibody reacts with Human.
- ReactivityHuman
- Storage Instruction-20°C
- UNSPSC12352203