![HepG2 whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with IGF2 antibody [N1C3] (GTX100453) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. HepG2 whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with IGF2 antibody [N1C3] (GTX100453) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX100453/GTX100453_44517_20230203_WB_Fraction_23020621_996.webp)
HepG2 whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with IGF2 antibody [N1C3] (GTX100453) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
IGF2 antibody [N1C3]
GTX100453
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman
TargetIGF2
Overview
- SupplierGeneTex
- Product NameIGF2 antibody [N1C3]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. ICC/IF: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration0.35 mg/ml
- ConjugateUnconjugated
- Gene ID3481
- Target nameIGF2
- Target descriptioninsulin like growth factor 2
- Target synonymsC11orf43, GRDF, IGF-II, PP9974, SRS3, insulin-like growth factor 2, T3M-11-derived growth factor, insulin-like growth factor 2 (somatomedin A), insulin-like growth factor type 2, preptin
- HostRabbit
- IsotypeIgG
- Protein IDP01344
- Protein NameInsulin-like growth factor 2
- Scientific DescriptionThis gene encodes a member of the insulin family of polypeptide growth factors that is involved in development and growth. It is an imprinted gene and is expressed only from the paternally inherited allele. It is a candidate gene for eating disorders. There is a read-through, INS-IGF2, which aligns to this gene at the 3 region and to the upstream INS gene at the 5 region. Alternatively spliced transcript variants, encoding either the same or different isoform, have been found for this gene. [provided by RefSeq]
- ReactivityHuman
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
![IGF2 antibody [N1C3] detects IGF2 protein at cytoplasm by immunofluorescent analysis. Sample: Hep G2 cells were fixed in ice-cold MeOH for 5 min. Green: IGF2 protein stained by IGF2 antibody [N1C3] (GTX100453) diluted at 1:500. Blue: Hoechst 33342 staining. IGF2 antibody [N1C3] detects IGF2 protein at cytoplasm by immunofluorescent analysis. Sample: Hep G2 cells were fixed in ice-cold MeOH for 5 min. Green: IGF2 protein stained by IGF2 antibody [N1C3] (GTX100453) diluted at 1:500. Blue: Hoechst 33342 staining.](https://www.genetex.com/upload/website/prouct_img/normal/GTX100453/GTX100453_39995_20170412_IFA_w_23060100_989.webp)

![WB analysis of HEK293 (1) and IGF2 (AA: 25-180)-hIgGFc transfected HEK293 (2) cell lysate using GTX60630 IGF2 antibody [8H1].](https://www.genetex.com/upload/website/prouct_img/normal/GTX60630/GTX60630_20170912_WB_w_23061123_506.webp)
![Mouse tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with IGF2 antibody [HL1979] (GTX637872) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX637872/GTX637872_T-44865_20230127_WB_M_placenta_23013122_169.webp)
![Mouse plasma (30 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with IGF2 antibody [HL1982] (GTX637875) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX637875/GTX637875_T-44865_20221230_WB_M_plasma_23010400_892.webp)
![HepG2 whole cell extract and conditioned medium (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with IGF2 antibody [HL2132] (GTX638104) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX638104/GTX638104_44998_20230331_WB_Fraction_23041023_266.webp)

