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Anti-IGF2R Antibody

144-60151
RayBiotech
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry
Product group Antibodies
TargetIGF2R
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Overview

  • Supplier
    RayBiotech
  • Product Name
    Anti-IGF2R Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    3.28 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID3482
  • Target name
    IGF2R
  • Target description
    insulin like growth factor 2 receptor
  • Target synonyms
    300 kDa mannose 6-phosphate receptor; cation-independent mannose-6-phosphate receptor; CD222; CI Man-6-P receptor; CI-M6PR; CIMPR; IGF-II receptor; insulin-like growth factor II receptor; M6P/IGF2 receptor; M6P/IGF2R; M6P-R; MPR 300; MPR1; MPR300; MPRI
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP11717
  • Protein Name
    Cation-independent mannose-6-phosphate receptor
  • Scientific Description
    IGF2R Polyclonal Antibody
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Raji cells(black) were fixed with 4% PFA for 10min at room temperature,permeabilized with 0.1% PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with IGF2R Polyclonal Antibody(bs-6670R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
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Figure 1. Western blot analysis of IGF2R using anti-IGF2R antibody (A00951). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human U87 whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: rat heart tissue lysates, Lane 5: rat PC-12 whole cell lysates, Lane 6: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IGF2R antigen affinity purified polyclonal antibody (Catalog # A00951) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IGF2R at approximately 274 kDa. The expected band size for IGF2R is at 274 kDa.
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