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Immunohistochemical staining of human spleen shows strong nuclear and cytoplasmic positivity in cells in red pulp.
Immunohistochemical staining of human spleen shows strong nuclear and cytoplasmic positivity in cells in red pulp.
Immunohistochemical staining of human spleen shows strong nuclear and cytoplasmic positivity in cells in red pulp.

Anti-IGSF8 Antibody

HPA075970
Atlas Antibodies
ApplicationsImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
ReactivityHuman
TargetIGSF8
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-IGSF8
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID93185
  • Target name
    IGSF8
  • Target description
    immunoglobulin superfamily member 8
  • Target synonyms
    CD316; CD81 partner 3; CD81P3; EWI2; EWI-2; glu-Trp-Ile EWI motif-containing protein 2; immunoglobulin superfamily member 8; KCT-4; keratinocytes-associated transmembrane protein 4; LIR-D1; PGRL; prostaglandin regulatory-like protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ969P0
  • Protein Name
    Immunoglobulin superfamily member 8
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of CD316/IGSF8 using anti-CD316/IGSF8 antibody (A06844-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human A431 whole cell lysates, Lane 4: human SH-SY5Y whole cell lysates, Lane 5: human HepG2 whole cell lysates, Lane 6: rat brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD316/IGSF8 antigen affinity purified polyclonal antibody (Catalog # A06844-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD316/IGSF8 at approximately 70 kDa. The expected band size for CD316/IGSF8 is at 65 kDa.
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