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Western Blot analysis of NIH-3T3 cells using CD316 Polyclonal Antibody.
Western Blot analysis of NIH-3T3 cells using CD316 Polyclonal Antibody.
Western Blot analysis of NIH-3T3 cells using CD316 Polyclonal Antibody.

IGSF8 Antibody

CSB-PA005881
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse
TargetIGSF8
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Overview

  • Supplier
    Cusabio
  • Product Name
    IGSF8 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID93185
  • Target name
    IGSF8
  • Target description
    immunoglobulin superfamily member 8
  • Target synonyms
    CD316, CD81P3, EWI-2, EWI2, KCT-4, LIR-D1, PGRL, immunoglobulin superfamily member 8, CD81 partner 3, glu-Trp-Ile EWI motif-containing protein 2, keratinocytes-associated transmembrane protein 4, prostaglandin regulatory-like protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ969P0
  • Protein Name
    Immunoglobulin superfamily member 8
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of CD316/IGSF8 using anti-CD316/IGSF8 antibody (A06844-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human A431 whole cell lysates, Lane 4: human SH-SY5Y whole cell lysates, Lane 5: human HepG2 whole cell lysates, Lane 6: rat brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD316/IGSF8 antigen affinity purified polyclonal antibody (Catalog # A06844-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD316/IGSF8 at approximately 70 kDa. The expected band size for CD316/IGSF8 is at 65 kDa.
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