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Anti-IKB beta Picoband antibody, PB9292, Western blotting All lanes: Anti IKB beta (PB9292) at 0.5ug/ml Lane 1: Mouse Kidney Tissue Lysate at 50ug Lane 2: RH35 Whole Cell Lysate at 40ug Lane 3: NRK Whole Cell Lysate at 40ug Lane 4: HELA Whole Cell Lysate at 40ug Predicted bind size: 38KD Observed bind size: 38KD
Anti-IKB beta Picoband antibody, PB9292, Western blotting All lanes: Anti IKB beta (PB9292) at 0.5ug/ml Lane 1: Mouse Kidney Tissue Lysate at 50ug Lane 2: RH35 Whole Cell Lysate at 40ug Lane 3: NRK Whole Cell Lysate at 40ug Lane 4: HELA Whole Cell Lysate at 40ug Predicted bind size: 38KD Observed bind size: 38KD
Anti-IKB beta Picoband antibody, PB9292, Western blotting All lanes: Anti IKB beta (PB9292) at 0.5ug/ml Lane 1: Mouse Kidney Tissue Lysate at 50ug Lane 2: RH35 Whole Cell Lysate at 40ug Lane 3: NRK Whole Cell Lysate at 40ug Lane 4: HELA Whole Cell Lysate at 40ug Predicted bind size: 38KD Observed bind size: 38KD

Anti-IKB beta/NFKBIB Antibody Picoband(r)

PB9292
Boster Bio
ApplicationsWestern Blot, ImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
TargetNFKBIB
100 ug
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Overview

  • Supplier
    Boster Bio
  • Product Name
    Anti-IKB Beta Picoband Antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: The detection limit for IKB beta is approximately 0.25ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    500 ug/ml
  • Gene ID4793
  • Target name
    NFKBIB
  • Target description
    NFKB inhibitor beta
  • Target synonyms
    ikappaBbeta; I-kappa-B-beta; IKBB; ikB-B; ikB-beta; NF-kappa-B inhibitor beta; NF-kappa-BIB; nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, beta; thyroid receptor-interacting protein 9; TR-interacting protein 9; TRIP9; TRIP-9
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ15653
  • Protein Name
    NF-kappa-B inhibitor beta
  • Scientific Description
    Boster Bio Anti-IKB beta/NFKBIB Antibody Picoband® catalog # PB9292. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of IkB Beta/NFKBIB using anti-IkB Beta/NFKBIB antibody (A05766-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human A549 whole cell lysates, Lane 5: rat testis tissue lysates, Lane 6: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IkB Beta/NFKBIB antigen affinity purified polyclonal antibody (Catalog # A05766-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IkB Beta/NFKBIB at approximately 45 kDa. The expected band size for IkB Beta/NFKBIB is at 38 kDa.
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