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ProductsBack/Anti-Interleukin-10 IL10 Antibody Picoband(r)
Figure. Western blot analysis of IL-10 using anti-IL-10 antibody (RP1014). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane : Recombinant Human IL-10 Protein 0.5ng After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL-10 antigen affinity purified polyclonal antibody (Catalog # RP1014) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL-10 at approximately 19KD. The expected band size for IL-10 is at 19KD.
Figure. Western blot analysis of IL-10 using anti-IL-10 antibody (RP1014). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane : Recombinant Human IL-10 Protein 0.5ng After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL-10 antigen affinity purified polyclonal antibody (Catalog # RP1014) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL-10 at approximately 19KD. The expected band size for IL-10 is at 19KD.
Figure. Western blot analysis of IL-10 using anti-IL-10 antibody (RP1014). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane : Recombinant Human IL-10 Protein 0.5ng After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL-10 antigen affinity purified polyclonal antibody (Catalog # RP1014) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL-10 at approximately 19KD. The expected band size for IL-10 is at 19KD.

Anti-Interleukin-10 IL10 Antibody Picoband(r)

RP1014
Boster Bio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetIL10
100 ug
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Overview

  • Supplier
    Boster Bio
  • Product Name
    Anti-Interleukin-10 IL10 Antibody Picoband(r)
  • Delivery Days Customer
    9
  • Application Supplier Note
    By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Applications Supplier
    ELI, IHP, WB, IHC
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    500 ug/ml
  • Gene ID3586
  • Target name
    IL10
  • Target description
    interleukin 10
  • Target synonyms
    CSIF, GVHDS, IL-10, IL10A, TGIF, interleukin-10, T-cell growth inhibitory factor, cytokine synthesis inhibitory factor
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP22301
  • Protein Name
    Interleukin-10
  • Scientific Description
    Boster Bio Anti-Interleukin-10 IL10 Antibody catalog # RP1014. Tested in ELISA, IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
  • Reactivity
    Human
  • Reactivity Supplier
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Zhao B, Gao WW, Liu YJ, et al. The role of glycogen synthase kinase 3 beta in brain injury induced by myocardial ischemia/reperfusion injury in a rat model of diabetes mellitus. Neural Regen Res. 2017,12(10):1632-1639. doi: 10.4103/1673-5374.217337
    Read this paper
  • Wang LQ, Yan XT, Yan CF, et al. Women with Recurrent Miscarriage Have Decreased Expression of 25-Hydroxyvitamin D3-1α-Hydroxylase by the Fetal-Maternal Interface. PLoS One. 2016,11(12):e0165589. doi: 10.1371/journal.pone.0165589
    Read this paper
  • Zhu XW, Zhu HZ, Zhu YQ, et al. Foxp3 expression in CD4(+)CD25(+)Foxp3(+) regulatory T cells promotes development of colorectal cancer by inhibiting tumor immunity. J Huazhong Univ Sci Technolog Med Sci. 2016,36(5):677-682. doi: 10.1007/s11596-016-1644-1
    Read this paper
  • Li G, Ren J, Wang G, et al. T2 enhances in situ level of Foxp3+ regulatory cells and modulates inflammatory cytokines in Crohn's disease. Int Immunopharmacol. 2014,18(2):244-8. doi: 10.1016/j.intimp.2013.12.014
    Read this paper
  • Wang F, Li H, Yang Z, et al. Expression of interleukin-10 in patients with adenomyosis. Fertil Steril. 2009,91(5):1681-5. doi: 10.1016/j.fertnstert.2008.02.164
    Read this paper

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Figure 1. Western blot analysis of IL10 using anti-IL10 antibody (A00021-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human U-87MG whole cell lysate, Lane 2: rat testis tissue lysates, Lane 3: mouse testis tissue lysates, Lane 4: mouse SP20 whole cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL10 antigen affinity purified polyclonal antibody (Catalog # A00021-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL10 at approximately 19KD. The expected band size for IL10 is at 21KD.
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