Anti-IL-1A [9H10]

Absolute Antibody

Anti-IL-1A [9H10]

9

This antibody binds mouse Interleukin-1 alpha, which is a cytokine constitutively present intracellularly in nearly all resting non-hematopoietic cells that plays an important role in inflammation and bridges the innate and adaptive immune systems. IL-1al

The original mouse IgG antibody binds mouse IL-1A with a binding affinity of Kd= 0.17 nM. The binding characterization of this antibody was done using ELISA. In vitro antibody potency was determined by its ability to inhibit IL-8 production by MRC-5 cells. This antibody was able to inhibit IL-8 production by MRC-5 cells with an IC50 of 2.00E-10M. This antibody was used in combination with 10G11 (anti- mouse IL-1B) antibody for the generation of anti-mouse IL-1alpha/beta DVD-Ig molecule (US7612181).

Functional Assay, ELISA

Research Use Only

Monoclonal

9H10

Il1a

interleukin 1 alpha

Il; IL-1 alpha; Il-1a; interleukin-1 alpha

Rat

IgG2a

Interleukin-1 alpha

This chimeric rat antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.

Mouse

-20°C,2°C to 8°C

12352203