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Anti-IL-34 [Ab2]

Ab00910-23.0
Absolute Antibody
ApplicationsOther Application
Product group Antibodies
TargetIL34
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Overview

  • Supplier
    Absolute Antibody
  • Product Name
    Anti-IL-34 [Ab2]
  • Delivery Days Customer
    7
  • Application Supplier Note
    This non-neutralising antibody binds to IL-34. A Fab fragment derived from this antibody has been used in crystallisation analysis of the structure of the active core of IL-34 (Ma et al, 2012).
  • Applications
    Other Application
  • Applications Supplier
    crystallisation
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    Ab2
  • Gene ID146433
  • Target name
    IL34
  • Target description
    interleukin 34
  • Target synonyms
    C16orf77; IL-34; interleukin-34
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ6ZMJ4
  • Protein Name
    Interleukin-34
  • Scientific Description
    This full-length, chimeric rabbit antibody was made using the variable domain sequences of the original Human scFv format, for improved compatibility with existing reagents, assays and techniques.
  • Reactivity Supplier
    Human, mouse
  • Reactivity Supplier Note
    This antibody was generated by phage display, in which murine IL-34 was coated onto immunoplates and used as the antigen for antibody phage library sorting.
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of IL34 using anti-IL34 antibody (A06903-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whle cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human SH-SY5Y whole cell lysates, Lane 4: human U87 whole cell lysates, Lane 5: human CACO-2 whole cell lysates, Lane 6: human U20S whole cell lysates, Lane 7: human HEK293 whole cell lysates, Lane 8: rat C6 whole cell lysates, Lane 9: mouse ANA-1 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL34 antigen affinity purified polyclonal antibody (Catalog # A06903-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL34 at approximately 39 kDa. The expected band size for IL34 is at 27 kDa.
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