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Anti-IL-6R [8C1D8B5]

Ab02464-23.0
Absolute Antibody
ApplicationsELISA
Product group Antibodies
ReactivityHuman
TargetIL6R
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Overview

  • Supplier
    Absolute Antibody
  • Product Name
    Anti-IL-6R [8C1D8B5]
  • Delivery Days Customer
    7
  • Application Supplier Note
    This antibody can be used to detect hIL6R in a sample (CN103059137).
  • Applications
    ELISA
  • Applications Supplier
    ELISA
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    8C1D8B5
  • Gene ID3570
  • Target name
    IL6R
  • Target description
    interleukin 6 receptor
  • Target synonyms
    CD126, HIES5, IL-1Ra, IL-6R, IL-6R-1, IL-6RA, IL6Q, IL6QTL, IL6RA, IL6RQ, gp80, interleukin-6 receptor subunit alpha, CD126 antigen, IL-6 receptor subunit alpha, membrane glycoprotein 80
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP08887
  • Protein Name
    Interleukin-6 receptor subunit alpha
  • Scientific Description
    This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
  • Reactivity
    Human
  • Reactivity Supplier
    Human
  • Reactivity Supplier Note
    The original antibody was raised by immunizing Blab/c mice with the IL-6R antigen.
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

FAQ

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Figure 1. Western blot analysis of IL6R using anti-IL6R antibody (A01425-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human A549 whole cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL6R antigen affinity purified polyclonal antibody (Catalog # A01425-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL6R at approximately 52KD. The expected band size for IL6R is at 52KD.
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