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Western blot analysis in human cell line THP-1.
Western blot analysis in human cell line THP-1.
Western blot analysis in human cell line THP-1.

Anti-IL17RA Antibody

AMAB91619
Atlas Antibodies
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman
TargetIL17RA
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-IL17RA
  • Delivery Days Customer
    4
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    CL10309
  • Conjugate
    Unconjugated
  • Gene ID23765
  • Target name
    IL17RA
  • Target description
    interleukin 17 receptor A
  • Target synonyms
    CANDF5, CD217, CDw217, IL-17RA, IL17R, IMD51, hIL-17R, interleukin-17 receptor A, IL-17 receptor A
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDQ96F46
  • Protein Name
    Interleukin-17 receptor A
  • Scientific Description
    Synthetic Peptide
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of IL17RA using anti-IL17RA antibody (A01311-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysate,Lane 2: human HepG2 whole cell lysate,Lane 3: human MCF-7 whole cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL17RA antigen affinity purified polyclonal antibody (Catalog # A01311-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL17RA at approximately 160KD. The expected band size for IL17RA is at 96KD.
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