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Lane 1: Raw264.7 cell lysates probed with IL17RA/CD217 Polyclonal Antibody, Unconjugated (bs-2606R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Raw264.7 cell lysates probed with IL17RA/CD217 Polyclonal Antibody, Unconjugated (bs-2606R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Raw264.7 cell lysates probed with IL17RA/CD217 Polyclonal Antibody, Unconjugated (bs-2606R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

IL17RA Polyclonal Antibody

BS-2606R
Bioss
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetIL17RA
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Overview

  • Supplier
    Bioss
  • Product Name
    IL17RA Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID23765
  • Target name
    IL17RA
  • Target description
    interleukin 17 receptor A
  • Target synonyms
    CANDF5, CD217, CDw217, IL-17RA, IL17R, IMD51, hIL-17R, interleukin-17 receptor A, IL-17 receptor A
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ96F46
  • Protein Name
    Interleukin-17 receptor A
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Sun B, Gao J, Shi W, et al. The interleukin-17 G-197A polymorphism is associated with cyclosporine metabolism and transplant rejection in liver transplant recipients. Pharmacogenomics. 2019,20(6):447-456. doi: 10.2217/pgs-2018-0198
    Read this paper
  • Bockerstett KA, Osaki LH, Petersen CP, et al. Interleukin-17A Promotes Parietal Cell Atrophy by Inducing Apoptosis. Cell Mol Gastroenterol Hepatol. 2018,5(4):678-690.e1. doi: 10.1016/j.jcmgh.2017.12.012
    Read this paper
  • Zhang DQ, Zhou CK, Chen SZ, et al. Identification of hub genes and pathways associated with bladder cancer based on co-expression network analysis. Oncol Lett. 2017,14(1):1115-1122. doi: 10.3892/ol.2017.6267
    Read this paper
  • Tang WJ, Tao L, Lu LM, et al. Role of T helper 17 cytokines in the tumour immune inflammation response of patients with laryngeal squamous cell carcinoma. Oncol Lett. 2017,14(1):561-568. doi: 10.3892/ol.2017.6253
    Read this paper

Datasheet

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Figure 1. Western blot analysis of IL17RA using anti-IL17RA antibody (A01311-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysate,Lane 2: human HepG2 whole cell lysate,Lane 3: human MCF-7 whole cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL17RA antigen affinity purified polyclonal antibody (Catalog # A01311-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL17RA at approximately 160KD. The expected band size for IL17RA is at 96KD.
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