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Anti-IL2RG Antibody

Anti-IL2RG Antibody

A101019
Antibodies.com
ApplicationsWestern Blot, ELISA
Product group Antibodies
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Overview

  • Supplier
    Antibodies.com
  • Product Name
    Anti-IL2RG Antibody
  • Delivery Days Customer
    7
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    Rabbit polyclonal antibody to IL2RG.
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of IL2 Receptor gamma/IL2RG using anti-IL2 Receptor gamma/IL2RG antibody (A00308-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human Raji whole cell lysates, Lane 4: human U-937 whole cell lysates, Lane 5: human HEK293 whole cell lysates, Lane 6: human HMY2.CIR whole cell lysates, Lane 7: human THP-1 whole cell lysates, Lane 8: rat RH-35 whole cell lysates, Lane 9: rat C6 whole cell lysates, Lane 10: mouse HEPA1-6 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL2 Receptor gamma/IL2RG antigen affinity purified polyclonal antibody (Catalog # A00308-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL2 Receptor gamma/IL2RG at approximately 65KD. The expected band size for IL2 Receptor gamma/IL2RG is at 65KD.
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