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Immunohistochemistry analysis in human urinary bladder and skeletal muscle tissues using AMAb91858 antibody. Corresponding IL33 RNA-seq data are presented for the same tissues.
Immunohistochemistry analysis in human urinary bladder and skeletal muscle tissues using AMAb91858 antibody. Corresponding IL33 RNA-seq data are presented for the same tissues.
Immunohistochemistry analysis in human urinary bladder and skeletal muscle tissues using AMAb91858 antibody. Corresponding IL33 RNA-seq data are presented for the same tissues.

Anti-IL33 Antibody

AMAB91858
Atlas Antibodies
ApplicationsImmunoCytoChemistry, ImmunoHistoChemistry
DownloadMSDS
Product group Antibodies
ReactivityHuman
TargetIL33
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-IL33
  • Delivery Days Customer
    12
  • Applications
    ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    CL13438
  • Conjugate
    Unconjugated
  • Gene ID90865
  • Target name
    IL33
  • Target description
    interleukin 33
  • Target synonyms
    C9orf26; DVS27; DVS27-related protein; IL1F11; interleukin-1 family member 11; interleukin-33; NFEHEV; NF-HEV; nuclear factor for high endothelial venules; nuclear factor from high endothelial venules
  • Host
    Mouse
  • Isotype
    IgG2b
  • Protein IDO95760
  • Protein Name
    Interleukin-33
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of IL33 using anti-IL33 antibody (A00113). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: recombinant human IL33 protein 1ng. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL33 antigen affinity purified polyclonal antibody (Catalog # A00113) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL33 at approximately 18KD. The expected band size for IL33 is at 18KD.
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