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Immunohistochemical staining of human colon shows nuclear positivity in endothelial cells.
Immunohistochemical staining of human colon shows nuclear positivity in endothelial cells.
Immunohistochemical staining of human colon shows nuclear positivity in endothelial cells.

Anti-IL33 Antibody

HPA024426
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetIL33
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-IL33
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID90865
  • Target name
    IL33
  • Target description
    interleukin 33
  • Target synonyms
    C9orf26, DVS27, IL1F11, NF-HEV, NFEHEV, interleukin-33, DVS27-related protein, interleukin-1 family member 11, nuclear factor for high endothelial venules, nuclear factor from high endothelial venules
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO95760
  • Protein Name
    Interleukin-33
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of IL33 using anti-IL33 antibody (A00113). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: recombinant human IL33 protein 1ng. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL33 antigen affinity purified polyclonal antibody (Catalog # A00113) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL33 at approximately 18KD. The expected band size for IL33 is at 18KD.
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