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Immunohistochemical staining of human Stomach shows strong cytoplasmic and membranous positivity in glandular cells.
Immunohistochemical staining of human Stomach shows strong cytoplasmic and membranous positivity in glandular cells.
Immunohistochemical staining of human Stomach shows strong cytoplasmic and membranous positivity in glandular cells.

Anti-IL7 Antibody

HPA019590
Atlas Antibodies
ApplicationsImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
TargetIL7
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-IL7
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID3574
  • Target name
    IL7
  • Target description
    interleukin 7
  • Target synonyms
    IL-7; interleukin-7
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP13232
  • Protein Name
    Interleukin-7
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of IL7 using anti-IL7 antibody (PB9521). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: K562 Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL7 antigen affinity purified polyclonal antibody (Catalog # PB9521) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL7 at approximately 75 kDa. The expected band size for IL7 is at 20 kDa.
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Immunofluorescence staining of U-251 cells using the Anti-IL7 monoclonal antibody, showing specific staining in the plasma membrane in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).
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Immunofluorescence staining of U-251 cells using the Anti-IL7 monoclonal antibody, showing specific staining in the plasma membrane in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).
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