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Immunohistochemical staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.
Immunohistochemical staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.
Immunohistochemical staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.

Anti-ILKAP Antibody

HPA004752
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetILKAP
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-ILKAP
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID80895
  • Target name
    ILKAP
  • Target description
    ILK associated serine/threonine phosphatase
  • Target synonyms
    ILKAP2, ILKAP3, PP2C-DELTA, PP2CD, PPM1O, integrin-linked kinase-associated serine/threonine phosphatase 2C, integrin-linked kinase associated phosphatase, protein phosphatase 2c, delta isozyme, protein phosphatase, Mg2+/Mn2+ dependent 1O
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9H0C8
  • Protein Name
    Integrin-linked kinase-associated serine/threonine phosphatase 2C
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of ILKAP using anti-ILKAP antibody (A08074-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human K562 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ILKAP antigen affinity purified polyclonal antibody (Catalog # A08074-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ILKAP at approximately 43 kDa. The expected band size for ILKAP is at 43 kDa.
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