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Western Blot analysis of HeLa cells using ILKAP Polyclonal Antibody
Western Blot analysis of HeLa cells using ILKAP Polyclonal Antibody
Western Blot analysis of HeLa cells using ILKAP Polyclonal Antibody

ILKAP Antibody

CSB-PA003037
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetILKAP
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Overview

  • Supplier
    Cusabio
  • Product Name
    ILKAP Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID80895
  • Target name
    ILKAP
  • Target description
    ILK associated serine/threonine phosphatase
  • Target synonyms
    ILKAP2; ILKAP3; integrin-linked kinase associated phosphatase; integrin-linked kinase-associated serine/threonine phosphatase 2C; PP2CD; PP2C-DELTA; PPM1O; protein phosphatase 2c, delta isozyme; protein phosphatase, Mg2+/Mn2+ dependent 1O
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9H0C8
  • Protein Name
    Integrin-linked kinase-associated serine/threonine phosphatase 2C
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of ILKAP using anti-ILKAP antibody (A08074-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human K562 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ILKAP antigen affinity purified polyclonal antibody (Catalog # A08074-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ILKAP at approximately 43 kDa. The expected band size for ILKAP is at 43 kDa.
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