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Immunofluorescent staining of human cell line MCF7 shows localization to nucleoli fibrillar center.
Immunofluorescent staining of human cell line MCF7 shows localization to nucleoli fibrillar center.
Immunofluorescent staining of human cell line MCF7 shows localization to nucleoli fibrillar center.

Anti-IMP4 Antibody

HPA066673
Atlas Antibodies
ApplicationsImmunoCytoChemistry
Product group Antibodies
ReactivityHuman
TargetIMP4
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-IMP4 Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID92856
  • Target name
    IMP4
  • Target description
    IMP U3 small nucleolar ribonucleoprotein 4
  • Target synonyms
    BXDC4, U3 small nucleolar ribonucleoprotein IMP4, IMP4 homolog, U3 small nucleolar ribonucleoprotein, IMP4, U3 small nucleolar ribonucleoprotein, homolog, U3 small nucleolar ribonucleoprotein protein IMP4, U3 snoRNP protein 4 homolog, U3 snoRNP protein IMP4, brix domain-containing protein 4
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ96G21
  • Protein Name
    U3 small nucleolar ribonucleoprotein protein IMP4
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of IMP4 using anti-IMP4 antibody (A10469-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: rat PC-12 whole cell lysates, Lane 4: mouse thymus tissue lysates, Lane 5: mouse RAW264.7 whole cell lysates, Lane 6: mouse EL-4 whole cell lysates, Lane 7: mouse Ana-1 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IMP4 antigen affinity purified polyclonal antibody (Catalog # A10469-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IMP4 at approximately 36 kDa. The expected band size for IMP4 is at 34 kDa.
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Immunofluorescent staining of human cell line MCF7 shows localization to nucleoli.
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