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Immunohistochemical staining of human thyroid gland shows distinct cytoplasmic and nuclear positivity in glandular cells.
Immunohistochemical staining of human thyroid gland shows distinct cytoplasmic and nuclear positivity in glandular cells.
Immunohistochemical staining of human thyroid gland shows distinct cytoplasmic and nuclear positivity in glandular cells.

Anti-ING5 Antibody

HPA042685
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetING5
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-ING5 Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID84289
  • Target name
    ING5
  • Target description
    inhibitor of growth family member 5
  • Target synonyms
    p28ING5, inhibitor of growth protein 5
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8WYH8
  • Protein Name
    Inhibitor of growth protein 5
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of ING5 using anti-ING5 antibody (A04974-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human PC-3 whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: monkey COS-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ING5 antigen affinity purified polyclonal antibody (Catalog # A04974-3) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ING5 at approximately 32 kDa. The expected band size for ING5 is at 28,32 kDa.
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