Bio-Connect

ING5 antibody [N2C1], Internal

GTX102482
GeneTex
ApplicationsWestern Blot
Product group Antibodies
TargetING5
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request

Overview

  • Supplier
    GeneTex
  • Product Name
    ING5 antibody [N2C1], Internal
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID84289
  • Target name
    ING5
  • Target description
    inhibitor of growth family member 5
  • Target synonyms
    inhibitor of growth protein 5; p28ING5
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8WYH8
  • Protein Name
    Inhibitor of growth protein 5
  • Scientific Description
    The protein encoded by this gene is similar to ING1, a tumor suppressor protein that can interact with TP53, inhibit cell growth, and induce apoptosis. This protein contains a PHD-finger, which is a common motif in proteins involved in chromatin remodeling. This protein can bind TP53 and EP300/p300, a component of the histone acetyl transferase complex, suggesting its involvement in TP53-dependent regulatory pathway. [provided by RefSeq]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

Related products

Immunofluorescent analysis of Hela cells using CSB-PA820185LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
Product group Antibodies
Cusabio
ING5 AntibodyCSB-PA820185LA01HU
ApplicationsImmunoFluorescence, ELISA
TargetING5
  • SizePrice
Product group Antibodies
Assay Genie
TargetING5
  • SizePrice
WB analysis of 293T cell lysate using GTX33266 ING5 antibody. Dilution : 1:1000 Loading : 25microg per lane
Product group Antibodies
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
TargetING5
  • SizePrice
Western blot analysis is shown using GeneTexs Affinity Purified anti-p28 ING5 antibody to detect over expressed Human ING5 present in cell extracts. This western blot shows reactivity with purified recombinant human ING5 protein. Comparison to a molecular weight marker (not shown) indicates a single band of ~36 kDa corresponding to the expected molecular weight for the recombinant protein. Approximately 10 microg of lysate was separated on a 4-20% Tris-Glycine gel by SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:1,500.Incubation was overnight at 4o C followed by washes and reaction with a 1:20,000 dilution of IRDye?800 conjugated Rb-a-Goat IgG [H&L] MXHu for 45 min at room temperature. IRDye?800 fluorescence image was captured using the OdysseyR Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.
Product group Antibodies
GeneTex
ApplicationsWestern Blot, ELISA
TargetING5
  • SizePrice
Immunohistochemical staining of human thyroid gland shows distinct cytoplasmic and nuclear positivity in glandular cells.
Product group Antibodies
Atlas Antibodies
ApplicationsImmunoHistoChemistry
TargetING5
  • SizePrice
Product group Antibodies
RayBiotech
ApplicationsImmunoFluorescence, Western Blot
TargetING5
  • SizePrice
Figure 1. Western blot analysis of ING5 using anti-ING5 antibody (A04974-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human PC-3 whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: monkey COS-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ING5 antigen affinity purified polyclonal antibody (Catalog # A04974-3) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ING5 at approximately 32 kDa. The expected band size for ING5 is at 28,32 kDa.
Product group Antibodies
Boster Bio
Anti-ING5 Antibody Picoband(r)A04974-3-CARRIER-FREE
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
TargetING5
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
ING5 AntibodyLS-C346332
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry
TargetING5
  • SizePrice
Product group Antibodies
Antibodies.com
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry
  • SizePrice