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Anti-INHBA Antibody

AMAB92029
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
DownloadMSDS
Product group Antibodies
ReactivityHuman, Rat
TargetINHBA
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-INHBA Antibody
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    CL15357
  • Conjugate
    Unconjugated
  • Gene ID3624
  • Target name
    INHBA
  • Target description
    inhibin subunit beta A
  • Target synonyms
    activin beta-A chain; EDF; erythroid differentiation factor; erythroid differentiation protein; follicle-stimulating hormone-releasing protein; FRP; FSH-releasing protein; inhibin beta A chain; inhibin beta A subunit; inhibin, beta A (activin A, activin AB alpha polypeptide); Inhibin, beta-1
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP08476
  • Protein Name
    Inhibin beta A chain
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human, Rat
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Inhibin beta A/INHBA using anti-Inhibin beta A/INHBA antibody (M06777). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U2OS whole cell lysates, Lane 2: human PC-3 whole cell lysates, Lane 3: human Hacat whole cell lysates, Lane 4: human U251 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Inhibin beta A/INHBA antigen affinity purified monoclonal antibody (M06777) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Inhibin beta A/INHBA at approximately 43 kDa. The expected band size for Inhibin beta A/INHBA is at 47 kDa.
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References
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Formalin-fixed and paraffin embedded human stomach labeled with Anti-Inhibin beta A/Activin A Polyclonal Antibody, Unconjugated (bs-1774R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
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TargetINHBA
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