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Anti-INHibin-alpha [15E11D5E11]

AB03405-10.3-BT
Absolute Antibody
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetINHA
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Overview

  • Supplier
    Absolute Antibody
  • Product Name
    Anti-INHibin-alpha [15E11D5E11]
  • Delivery Days Customer
    9
  • Application Supplier Note
    The antibody was used to stain ovarian sex cord stromal tumor in immunohistochemical analysis (CN114075281A).
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    15E11D5E11
  • Gene ID3623
  • Target name
    INHA
  • Target description
    inhibin subunit alpha
  • Target synonyms
    inhibin alpha chain, A-inhibin subunit, inhibin alpha subunit
  • Host
    Human
  • Isotype
    IgG1
  • Protein IDP05111
  • Protein Name
    Inhibin alpha chain
  • Scientific Description
    This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG1 format for improved compatibility with existing reagents assays and techniques.
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of INHA using anti-INHA antibody (A02413-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: rat brain tissue lysates, Lane 4: mouse spleen tissue lysates, Lane 5: mouse sp2/0 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-INHA antigen affinity purified polyclonal antibody (Catalog # A02413-3)at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for INHA at approximately 45KD. The expected band size for INHA is at 45KD.
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