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Anti-INPP4b [N171/17.4]

AB02144-10.3-BT
Absolute Antibody
ApplicationsWestern Blot, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman
TargetINPP4B
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Overview

  • Supplier
    Absolute Antibody
  • Product Name
    Anti-INPP4b [N171/17.4]
  • Delivery Days Customer
    9
  • Application Supplier Note
    This antibody is recommended for the detection and analysis of INPP4b by western blot and immunocytochemistry. For instance, the mouse version of this antibody was used to detect INPP4b by western blot in extracts of COS cells transiently transfected with GFP-tagged INPP4b or untagged Kv2.1 plasmid.
  • Applications
    Western Blot, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    N171/17.4
  • Gene ID8821
  • Target name
    INPP4B
  • Target description
    inositol polyphosphate-4-phosphatase type II B
  • Target synonyms
    inositol polyphosphate 4-phosphatase type II, inositol polyphosphate 4-phosphatase II; 4-phosphatase II, inositol polyphosphate-4-phosphatase, type II, 105kDa, type II inositol 3,4-bisphosphate 4-phosphatase
  • Host
    Human
  • Isotype
    IgG1
  • Protein IDO15327
  • Protein Name
    Inositol polyphosphate 4-phosphatase type II
  • Scientific Description
    This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of INPP4B using anti-INPP4B antibody (M06336). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-INPP4B antigen affinity purified monoclonal antibody (Catalog # M06336) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for INPP4B at approximately 105 kDa. The expected band size for INPP4B is at 105,107 kDa.
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