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Anti-Insulin degrading enzyme/IDE Antibody

ORB2612464
Biorbyt
ApplicationsFlow Cytometry, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetIDE
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Overview

  • Supplier
    Biorbyt
  • Product Name
    Anti-Insulin degrading enzyme/IDE Antibody
  • Delivery Days Customer
    10
  • Application Supplier Note
    Western blot, 0.1-0.5microg/ml Immunohistochemistry (Paraffin-embedded Section), 0.5-1microg/ml Immunohistochemistry (Frozen Section), 0.5-1microg/ml Immunocytochemistry, 0.5-1microg/ml Flow Cytometry (Fixed), 1-3microg/1x106 cells ELISA, 0.1-0.5microg/ml. Add 0.2ml of distilled water will yield a concentration of 500ug/ml
  • Applications
    Flow Cytometry, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Applications Supplier
    ELISA, FC, ICC, IHC, WB
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    500 ug/ml
  • Conjugate
    Cy3
  • Gene ID3416
  • Target name
    IDE
  • Target description
    insulin degrading enzyme
  • Target synonyms
    INSULYSIN, insulin-degrading enzyme, Abeta-degrading protease, insulin protease, insulinase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP14735
  • Protein Name
    Insulin-degrading enzyme
  • Scientific Description
    Anti-Insulin degrading enzyme/IDE Antibody. Tested in ELISA, Flow Cytometry, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of IDE using anti-IDE antibody (M01358). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human U251 whole cell lysates, Lane 3: human U2OS whole cell lysates, Lane 4: human SH-SY5Y whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat C6 whole cell lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse Neuro-2a whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IDE antigen affinity purified monoclonal antibody (M01358) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IDE at approximately 118 kDa. The expected band size for IDE is at 118 kDa.
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