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Anti-IQGAP1 Antibody

144-61421
RayBiotech
ApplicationsWestern Blot
Product group Antibodies
TargetIQGAP1
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Overview

  • Supplier
    RayBiotech
  • Product Name
    Anti-IQGAP1 Antibody
  • Delivery Days Customer
    16
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID8826
  • Target name
    IQGAP1
  • Target description
    IQ motif containing GTPase activating protein 1
  • Target synonyms
    HUMORFA01; p195; ras GTPase-activating-like protein IQGAP1; RasGAP-like with IQ motifs; SAR1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP46940
  • Protein Name
    Ras GTPase-activating-like protein IQGAP1
  • Scientific Description
    IQGAP1 Polyclonal Antibody
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of IQGAP1 using anti-IQGAP1 antibody (A01603-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U87 whole cell lysates, Lane 2: human SW620 whole cell lysates, Lane 3: human U20S whole cell lysates, Lane 4: human HEK293 whole cell lysates, Lane 5: human K562 whole cell lysates, Lane 6: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IQGAP1 antigen affinity purified polyclonal antibody (Catalog # A01603-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IQGAP1 at approximately 195 kDa. The expected band size for IQGAP1 is at 189 kDa.
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