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Western Blot analysis of HeLa cells using IQGAP1 Polyclonal Antibody
Western Blot analysis of HeLa cells using IQGAP1 Polyclonal Antibody
Western Blot analysis of HeLa cells using IQGAP1 Polyclonal Antibody

IQGAP1 Antibody

CSB-PA003059
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse
TargetIQGAP1
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Overview

  • Supplier
    Cusabio
  • Product Name
    IQGAP1 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID8826
  • Target name
    IQGAP1
  • Target description
    IQ motif containing GTPase activating protein 1
  • Target synonyms
    HUMORFA01; p195; ras GTPase-activating-like protein IQGAP1; RasGAP-like with IQ motifs; SAR1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP46940
  • Protein Name
    Ras GTPase-activating-like protein IQGAP1
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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U87MG cells were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with IQGAP1 Polyclonal Antibody(bs-1109R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
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Figure 1. Western blot analysis of IQGAP1 using anti-IQGAP1 antibody (A01603-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U87 whole cell lysates, Lane 2: human SW620 whole cell lysates, Lane 3: human U20S whole cell lysates, Lane 4: human HEK293 whole cell lysates, Lane 5: human K562 whole cell lysates, Lane 6: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IQGAP1 antigen affinity purified polyclonal antibody (Catalog # A01603-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IQGAP1 at approximately 195 kDa. The expected band size for IQGAP1 is at 189 kDa.
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