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Anti-IQGAP2 [A2]

AB02625-2.3-BT
Absolute Antibody
ApplicationsImmunoFluorescence
Product group Antibodies
ReactivityHuman, Mouse
TargetIQGAP2
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Overview

  • Supplier
    Absolute Antibody
  • Product Name
    Anti-IQGAP2 [A2]
  • Delivery Days Customer
    9
  • Application Supplier Note
    Aliquots of chronically infected or uninfected N2a cells were used for immunofluorescence with the mouse version of this antibody. This was done to research genes associated with prion propagation (Marbiah et al, 2014; pmid:24843046).
  • Applications
    ImmunoFluorescence
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    A2
  • Gene ID10788
  • Target name
    IQGAP2
  • Target description
    IQ motif containing GTPase activating protein 2
  • Target synonyms
    ras GTPase-activating-like protein IQGAP2
  • Host
    Mouse
  • Isotype
    IgG2a
  • Protein IDQ13576
  • Protein Name
    Ras GTPase-activating-like protein IQGAP2
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of IQGAP2 using anti-IQGAP2 antibody (A04787-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela cell lysates, Lane 2: human placenta tissue lysates, Lane 3: human HepG2 cell lysates, Lane 4: mouse stomach tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IQGAP2 antigen affinity purified polyclonal antibody (Catalog # A04787-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IQGAP2 at approximately 181KD. The expected band size for IQGAP2 is at 181KD.
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