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Immunohistochemical staining of human pancreas shows moderate nuclear positivity in exocrine glandular cells.
Immunohistochemical staining of human pancreas shows moderate nuclear positivity in exocrine glandular cells.
Immunohistochemical staining of human pancreas shows moderate nuclear positivity in exocrine glandular cells.

Anti-IRF2BPL Antibody

HPA050862
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetIRF2BPL
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-IRF2BPL Antibody
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID64207
  • Target name
    IRF2BPL
  • Target description
    interferon regulatory factor 2 binding protein like
  • Target synonyms
    C14orf4, EAP1, NEDAMSS, probable E3 ubiquitin-protein ligase IRF2BPL, E3 ubiquitin-protein ligase IRF2BPL, enhanced at puberty 1, enhanced at puberty protein 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9H1B7
  • Protein Name
    Probable E3 ubiquitin-protein ligase IRF2BPL
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of IRF2BPL using anti-IRF2BPL antibody (A10706). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human PC-3 whole cell lysates, Lane 2: human SH-SY5Y whole cell lysates, Lane 3: human MCF-7whole cell lysates, Lane 4: human RT4 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IRF2BPL antigen affinity purified polyclonal antibody (Catalog # A10706) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IRF2BPL at approximately 95 kDa. The expected band size for IRF2BPL is at 83 kDa.
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