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Immunohistochemical staining of human lymph node shows weak cytoplasmic positivity in germinal center cells.
Immunohistochemical staining of human lymph node shows weak cytoplasmic positivity in germinal center cells.
Immunohistochemical staining of human lymph node shows weak cytoplasmic positivity in germinal center cells.

Anti-IRF3 Antibody

HPA004895
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetIRF3
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-IRF3
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID3661
  • Target name
    IRF3
  • Target description
    interferon regulatory factor 3
  • Target synonyms
    IIAE7, interferon regulatory factor 3
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ14653
  • Protein Name
    Interferon regulatory factor 3
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of IRF3 using anti-IRF3 antibody (A00165-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human PC-3 whole cell lysates, After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IRF3 antigen affinity purified polyclonal antibody (Catalog # A00165-4) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IRF3 at approximately 55KD. The expected band size for IRF3 is at 55KD.
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